Nikolaos Sideris

PIPS Host Organisation: Stratosvir Ltd

PIPS Project Title: Development of new oncolytic virus production technology

During his placement at Stratosvir Ltd, Nikos explored onboarding and adapting methodology reported in academic papers which may be able to accelerate the company’s research pipeline, potentially improving speed, reproducibility, and accessibility of experimental research workflows. The primary objective of the project was to optimise viral production processes that could increase throughput and enable rapid iteration. Through method refinement and process optimisation, Nikos successfully accelerated the production workflow and generated three engineered viruses, exceeding the initial target of producing a single virus within the placement period.

In delivering on a stretch goal, Nikos also redeveloped an existing assay that enables viral viability assessment without reliance on specialised reagents or proprietary equipment. By adapting this protocol for easy use with available laboratory materials, he reduced cost, simplified execution, and improved scalability. This modification allowed the assay to be implemented more broadly across the team and supported faster decision-making during construct screening.

In addition to experimental development, Nikos focused on knowledge transfer and reproducibility. He authored comprehensive Standard Operating Procedures (SOPs) for both the optimised viral production workflow and the adapted viability assay. These documents provide clear, step-by-step guidance to ensure consistency, facilitate onboarding of new staff, and enable sustainable adoption of the improved methods beyond the placement.

Overall, his work delivered measurable gains in efficiency, reduced technical barriers, and established robust documentation to support ongoing development. These contributions directly supported Stratosvir’s goal of streamlining production of research viruses and enhancing experimental throughput while maintaining reliability and accessibility.

During his PIPS placement, Nikos strengthened technical skills in applied virology, data analysis, and experimental evaluation while gaining hands-on experience with industry R&D workflows. He also developed skills in project management, teamwork, communication, and adaptability, and expanded his professional network, gaining valuable insight into scientific careers and building confidence for future roles.